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  7. Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)

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Fig. 1: Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha Mouse Monoclonal Antibody (4C6-H8).

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)
Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)

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Fig. 1: Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha Mouse Monoclonal Antibody (4C6-H8).
Fig. 2: Immunofluorescence staining of paraformaldehyde-fixed HePG2 cells with TNF alpha Mouse Monoclonal Antibody (4C6-H8) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).

Product code: 36-3278

Clone name : 4C6-H8
Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human, Mouse, Rat

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgM, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : TNF
Gene ID : 7124
Uniprot ID : P01375
Alternative Name : APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor
Immunogen Information : A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF- , conjµgated to thyroglobulin
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Althoµgh it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml);Immunohistochemistry (Formalin-fixed) (2-4µg/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)

Product code: 36-3278
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