Human IL-13 (Interleukin 13) Pre-Coated ELISA Kit
Fig-1:Human IL-13 Standard Curve
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| Amount : | 1 × 96 Tests |
| Content : | 1 × 96 well Format (96 tests) |
| Storage condition : | Please refer to the Manual |
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-13 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-13 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-13 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-13 can be calculated using the standard curve.
This immunoassay kit allows for the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma and other biological fluids.
Detection Range: 15.6 – 1000 pg/ml
Sensitivity: < 9.4 pg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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