|Amount :||10 µg|
|Purification :||Greater than 95.0% as determined by SDS-PAGE.|
|Content :||MIF T.Vaginalis filtered (0.4 µm) and lyophilized from 0.5mg/ml solution in 20 mM Tris buffer and 50 mM NaCl, pH 7.5.
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. MIF T.Vaginalis is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
|Storage condition :||Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.|
|AA sequence :||MPALVIKTNA KFTEEEKSKA TEELGNIVSK VLGKPISYVM VTLEDGVAVR FGGSDEKAAF MSLMSIGGLN RAVNKRASAA LTKWFTDHGF QGDRIYIVFN PKSAEDWGFN GDTFAHHHHH H.|
|Alternative Name :||Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor), Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase, GLIF, MMIF, GIF, Macrophage Migration Inhibitory Factor, Glycosylation-Inhibiting Factor, EC 184.108.40.206, EC 220.127.116.11, Macrophage migration inhibitory factor.|
Filtered White lyophilized (freeze-dried) powder.
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
MIF T.Vaginalis Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 1-115) containing 121 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 13.4kDa (calculated).