Monoclonal Antibody to Alpha-II spectrin (Clone: 3D7)
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with mouse mAb to αII-Spectrin,(34-1108), dilution 1:500 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2),(34-1064), dilution 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains membranes of neuronal cell body, axons and their dendrites, while MAP2 antibody labels dendrites and perikarya of mature neurons only.
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Mouse, IgG1 |
| Content : | Supplied as an aliquot of affinity purified antibody at 1mg/ml in PBS, 50% glycerol, 5mM NaN3 |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | SPTAN1 |
| Gene ID : | 6709 |
| Uniprot ID : | Q13813 |
| Alternative Name : | Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit |
| Immunogen Information : | Recombinant construct containing the 13th, 14th and 15th spectrin repeats of human Alpha -II spectrin (amino acids 1398-1685) expressed in and purified from E. coli. |
WB: 1:3,000. IF/ICC: 1:500.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm |
| Post transnational modification: | Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. |
| BioGrid: | 112587. 179 interactions. |
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