Monoclonal Antibody to CD44 / HCAM Std.(Clone : SPM544)
|Purification :||Affinity Chromatography|
|Content :||100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Functional Studies (Order Ab without BSA & Azide); Western Blot (0.5-1µg/ml); Immunohistology (Formalin-fixed) (0.25-0.5µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cell membrane, Cell projection|
|Post transnational modification:||Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.|
|Tissue Specificity:||Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.|
|BioGrid:||107398. 132 interactions.|