Monoclonal Antibody to CD69 (Clone: ABM39A4) BSA Free
Fig-1: Cell surface FLOW analysis of hCD69 (10-4110) in PBMC treated 3 days with PMA + Ionomycin (Lymphocytes gated) using 0.5 µgantibody per 10^6 cells. Green represents PBMC W/O treatment, red represents PBMC with treatment. Goat anti-mouse PE conjugated secondary antibody was used (ABEOMICS).
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|Amount :||100 µg|
|Isotype :||Mouse IgG2a Kappa|
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.|
CD69 is a type II transmembrane protein of the C-type lectin family and its gene is located within the natural killer gene complex. In healthy subjects, CD69 is not detected in peripheral blood lymphocytes, but is expressed on small subsets of T and B cells in peripheral lymphoid tissues. CD69 gene maps at human chromosome 12, and behaves as an early activation gene that contains responsive elements for the transcription factors NF-kappaB, ERG-1 and AP-1. This protein acts as a leukocyte activation marker, and is involved in the activation of different leukocyte subsets as well as in the pathogenesis of chronic inflammation.
Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.