Monoclonal Antibody to DNMT3a (Clone: ABM13G4 )
|Amount :||100 µg|
|Isotype :||Mouse IgG1, Kappa|
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.|
Dnmt3a belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. Dnmt3a together with other methyltransferases conducts de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring. Dnmt3a also directs a metabolic program by repressing key genes to enable the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been found in several cancers including AML (Acute Myeloid Leukemia).
WB: 2-4 µg/ml, FACS: 0.5-1 µg/10^6
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Nucleus, Cytoplasm|
|Post transnational modification:||Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.|
|Tissue Specificity:||Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.|
|BioGrid:||108125. 53 interactions.|