Monoclonal Antibody to MBD1 (Clone: ABM15H2)

Product code: 10-7007

Clone name : ABM15H2
Clonality : Monoclonal
Application : IHC, FACS, WB
Reactivity : Human

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Available Pack Size(s)

  •   25 µg

  •  100 µg

  • $110.00 

  • $290.00 

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Shipping Info:

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1 Kappa
Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : MBD1
Gene ID : 4152
Uniprot ID : Q9UIS9
Alternative Name : MBD1, CXXC3, PCM1
Immunogen Information : A partial length recombinant MBD1 protein (amino acids 291-586) was used as the immunogen for this antibody.

MBD1 (Methyl-CpG-Binding Domain Protein 1) protein is a primary candidate for the readout of DNA methylation as it recruits chromatin remodelers, histone deacetylases and methylases to methylated DNA associated with gene repression. This protein, a member of a transcriptional repressor family MBD, is predominantly expressed in neurons. MBD protein binding requires both functional MBD domains and methyl-CpGs; however, some MBD proteins also bind unmethylated DNA and active regulatory regions via alternative regulatory domains or interaction with the NuRD/Mi-2 (Nucleosome Remodeling Deacetylase) complex members. The CXXC3 domain of MBD1 makes it a unique member of the MBD family due to its affinity to unmethylated DNA. MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex. It also plays an important role in disease progression, contributes to the drug resistance of PC cells; however, the mechanism underlying the drug resistance endowed by MBD1 remains unknown.

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, Facs analysis:.0.5-1 µg/10^6 Cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Nucleus matrix, Nucleus speckle, Chromosome
Post transnational modification: Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Tissue Specificity: Widely expressed.
BioGrid: 110322. 30 interactions.
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