Monoclonal Antibody to MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule)(Clone : C146/634)
|Purification :||Affinity Chromatography|
|Content :||100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1.0µg/ml); Immunohistology (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT); ((Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Tissue Specificity:||Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.|
|BioGrid:||110332. 15 interactions.|