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  7. Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)

Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)

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Fig-1: Western Blot of A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)

Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)
Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)

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Fig-1: Western Blot of A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)
Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (NCL/902).
Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (NCL/902).
Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).
Formalin-fixed, paraffin-embedded human Bladder carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
Flow Cytometry of Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (NCL/902).
HeLa Cells stained with AF488 labeled Nucleolin Monoclonal Antibody (NCL/902) Green: AF488-labeled Ab. Blue: DAPI.

Product code: 36-1503

Clone name : NCL/902
Clonality : Monoclonal
Application : IHC, FACS, WB, IF
Reactivity : Human

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100 µg
$459.00 

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Download TDS MSDS

  • Product Info
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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1 Kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : NCL
Gene ID : 4691
Uniprot ID : P19338
Alternative Name : NCL
Immunogen Information : Recombinant human NCL protein

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Western Blot (0.5-1µg/ml); Immunohistology (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Cytoplasm
Post transnational modification: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
BioGrid: 110771. 358 interactions.
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Monoclonal Antibody to Nucleolin (Marker of Human Cells)(Clone : NCL/902)(Clone : NCL/902)

Product code: 36-1503
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