Monoclonal Antibody to RIG-I (Clone: ABM40B5)
RIG-I or Probable ATP-dependent RNA helicase DDX58 or simply DDX58 is a RNA helicase enzyme with two isoforms (106 and 101 kDa). RIG-I belongs to the DEAD/H box family and is involved in several cellular responses including RNA binding and alteration of RNA secondary structure. RIG-1 acts as sensor of viral nucleic acids thus activating a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Generally it remains as a monomer but upon activation by viral dsRNA it homomultimerizes and interacts with mitochondria antiviral signaling protein (MAVS/IPS1) which activating a cascade of reactions to produce specific interferon. RIG-1 is mostly expressed in vascular smooth cells.
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.|
Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, Flowcytometric analysis: 0.5-1µg/10^6 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cytoplasm, Cell projection, Cytoplasm, Cell junction|
|Post transnational modification:||Sumoylated, probably by MUL1; inhibiting its polyubiquitination.|
|Tissue Specificity:||Present in vascular smooth cells (at protein level).|
|BioGrid:||117121. 43 interactions.|