Monoclonal Antibody to TLR4 / CD284 (Clone: ABM19C4)
TLR4 (Toll-like receptor-4) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. It primarily mediates cellular signaling induced by Gram negative bacteria. TLR4 is highly specific for LPS and it is associated with CD14, a co-receptor for LPS. The downstream signaling pathway of TLR4 leads to the activation of NF-kappaB through myeloid differentiation protein (MyD88) and IL-1 receptor-associated kinase in various cell types. Keratinocytes express TLR4 at the mRNA and protein levels whereas TLR4 are also present in the normal human epidermis in vivo and their expression is regulated by microbial components. TLR4 is expressed on CD4+ T cells as well, the functional significance of which is unclear.
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.|
Immunohistochemical analysis: 5 µg/ml; FACS analysis: 2-4 µg/10^6
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
1. Parida R. Human MOSPD2: A bacterial Lmb mimicked auto-antigen is involved in immune infertility. Journal of Translational Autoimmunity. 2019 Apr;100002 (1). https://doi.org/10.1016/j.jtauto.2019.100002.(Immunofluorescence)
|Subcellular location:||Cell membrane|
|Post transnational modification:||N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.|
|Tissue Specificity:||Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.|
|BioGrid:||112954. 31 interactions.|