Monoclonal Antibody to TLR4 (Clone : 3C3)
The monoclonal antibody 10-3522 reacts with human Toll-like receptor 4 (TLR4, CD284). TLRs belong to a family of proteins that specifically recognize and sense microbial products. They are highly conserved throughout evolution. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. TLRs identified as type I transmembrane signalling receptors act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. The monoclonal antibody 3C3 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.
|Content :||0.5 mg, 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.|
|Storage condition :||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
|Subcellular location:||Cell membrane|
|Post transnational modification:||N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.|
|Tissue Specificity:||Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.|
|BioGrid:||112954. 31 interactions.|