Mouse Monoclonal Antibody to PCNA (Clone : 4C10G3)(Discontinued)
Figure-1 : Western blot analysis of PCNA Antibody (Clone: 4C10G3) at 1 µg/ml on 1: Hela cell lysate, 2: NIH/3T3 cell lysate.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Mouse IgG1, k |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
The proliferating cell nuclear antigen (PCNA) is a 36 kDa nuclear protein associated with the cell cycle. PCNA was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase ?. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation. Mouse Anti-PCNA Monoclonal Antibody is developed in mouse using recombinant human PCNA protein. Mouse Anti-PCNA is highly purified from mouse ascites by protein A chromatography and is supplied as 40 ?g aliquot.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus |
| Post transnational modification: | Methylated on glutamate residues by ARMT1/C6orf211. |
| BioGrid: | 111142. 314 interactions. |
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