Mouse Monoclonal Antibody to RAD51 (Clone: 1690CT417.18.80)(Discontinued)
Figure 1: All lanes : Anti-RAD51 Antibody (10-6635) at 1:2000-1:4000 dilution with Lane 1: Hela whole cell lysate, Lane 2: Jurkat whole cell lysate, Lane 3: K562 whole cell lysate, Lane 4: A431 whole cell lysate, Lane 5: Ramos whole cell lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa.
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| Format : | Purified |
| Amount : | 200 µl |
| Isotype : | Mouse IgG2b,Kappa |
| Purification : | Protein G Chromatography |
| Content : | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Storage condition : | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
WB~1:2000-1:4000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Cytoplasm, Cytoplasm, Mitochondrion matrix, Cytoplasm |
| Post transnational modification: | Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. |
| Tissue Specificity: | Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. |
| BioGrid: | 111825. 145 interactions. |
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