Mouse Monoclonal Antibody to Phosphotyrosine (Clone : 5E10E1)(Discontinued)
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|Amount :||100 µg|
|Isotype :||Mouse IgG1, k|
|Purification :||Protein A chromatography|
|Content :||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Storage condition :||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Immunogen Information :||Phosphotyrosine containing carrier protein|
Phosphotyrosine is a tyrosine residue covalently bound to a phosphate via its hydroxyl group. A tyrosine kinase catalyzes the transfer of a phosphate from ATP to a tyrosine residue on a protein substrate. Tyrosine phosphorylation plays a key role in intracellular signaling and cancer development. Many tyrosine kinases are drug targets for different forms of cancer. Phosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuable tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery. Phosphotyrosine Antibody (5E10), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with phosphotyrosine containing carrier protein.
Western Blot: 1-2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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