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  7. Anti-beta-tubulin / TUBB Monoclonal Antibody (Clone:TU-13)

Anti-beta-tubulin / TUBB Monoclonal Antibody (Clone:TU-13)

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Figure 1: Western blotting analysis of porcine brain lysate using anti-beta tubulin (TU-13) purified.

Anti-beta-tubulin / TUBB Monoclonal Antibody (Clone:TU-13)

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Product code: 30-1306

Clone name : TU-13
Clonality : Monoclonal
Application : WB
Reactivity : Human

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0.1 mg
$365.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgM
Purification : Purified by precipitation and chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : TUBB
Gene ID : 203068
Uniprot ID : P07437
Alternative Name : Tubulin beta-5 chain, TUBB5
Immunogen Information : beta-tubulin from porcine brain

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

Western Blotting Recommended dilution: 1-2 µg/ml
Positive control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa human cell line, EL4 mouse cell line, MCF7 human cell line

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cell junction, Lateral cell membrane, Cytoplasm, Cell junction, Cytoplasm
Tissue Specificity: Ubiquitously expressed.
BioGrid: 112767. 61 interactions.
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Anti-beta-tubulin / TUBB Monoclonal Antibody (Clone:TU-13)

Product code: 30-1306
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