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  7. Recombinant Mouse Monoclonal Antibody to Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)(Clone : rWT1/857)

Recombinant Mouse Monoclonal Antibody to Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)(Clone : rWT1/857)

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Figure 1: Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm's Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).

Recombinant Mouse Monoclonal Antibody to Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)(Clone : rWT1/857)
Recombinant Mouse Monoclonal Antibody to Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)(Clone : rWT1/857)

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Figure 1: Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm's Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).
Figure 2: SDS-PAGE Analysis of Purified Wilm's Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).

Product code: 12-1011

Clone name : rWT1/857
Clonality : Monoclonal
Application : IHC, FACS, IF
Reactivity : Human, Mouse, Rat

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  •  100 µg

  • $199.00 

  • $459.00 

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1, kappa
Purification : Purified Ab with BSA and Azide at 200ug/ml
Content : 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : WT1
Gene ID : 7490
Uniprot ID : P19544
Alternative Name : WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1
Immunogen Information : Recombinant full-length human WT1 protein

Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

MW : 47-55kDa; Positive Control : K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus
Tissue Specificity: Expressed in the kidney and a subset of hematopoietic cells.
BioGrid: 113327. 55 interactions.
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Recombinant Mouse Monoclonal Antibody to Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)(Clone : rWT1/857)

Product code: 12-1011
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