Recombinant SARS-CoV-2 NSP8 (C-His)
|Amount :||50 µg|
|Content :||Supplied as a 0.2 um filtered solution of 20mM Tris, 150mM NaCl, 10%Glycerol, 4mM MgCl2, pH8.0|
|AA sequence :||Recombinant SARS-CoV-2 NSP8 is produced by our E.coli expression system and the target gene encoding Ala1-Gln198 is expressed with a 8His tag at the C-terminus.|
|Alternative Name :||Non-Structural Protein 8; nsp8|
The replication of coronavirus is operated by a set of non-structural proteins (nsps) encoded by open reading frame 1a (ORF1a) and ORF1ab in its genome. NSP8 is a component of the coronavirus replicase polyprotein that forms a replication complex. It can form a dimer with NSP7 and subsequently assembles into a large hexadecameric structure, which participates in viral replication by acting as a primase. NSP8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that is necessary for producing primers utilized by NSP12 which is normally accepted to be the RdRp for SARS-CoV.