Anti-Basic Cytokeratins Monoclonal Antibody (Clone:AE3)
Figure-1: Western blotting analysis of human basic cytokeratins using mouse monoclonal antibody AE3 on lysates of MCF-7 cell line and Molt-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-basic cytokeratins monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for basic cytokeratins at approximately 40-55 kDa.
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|Amount :||0.1 mg|
|Isotype :||Mouse IgG1|
|Purification :||Purified by protein-A affinity chromatography|
|Storage condition :||Store at 2-8°C. Do not freeze.|
|Immunogen Information :||Human epidermal keratins|
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Western blotting: Recommended dilution: 1-2 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Tissue Specificity:||Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.|
|BioGrid:||110064. 136 interactions.|