Figure 1: ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 μg/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in a dose-dependent manner, but with different dynamics when the biotinylated antibody was detected by anti-biotin mAb/goat anti-mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in a dose-dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).
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|Amount :||0.1 mg|
|Isotype :||Mouse IgG2a kappa|
|Purification :||Purified by protein-A affinity chromatography.|
|Content :||Concentration: 1 mg/ml
Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
|Storage condition :||Store at 2-8°C. Do not freeze.|
|Alternative Name :||vitamin H, vitamin B7|
|Immunogen Information :||biotinylated limulus hemocyanin|
Specificity: Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.
FC (QC tested), ELISA, IP, MC (CyTOF)
For Research Use Only. Not for use in diagnostic/therapeutics procedures.