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  7. Rabbit Polyclonal Antibody to HSP70(Discontinued)

Rabbit Polyclonal Antibody to HSP70(Discontinued)

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Figure-1 : Western blot analysis of HSP70 Antibody at 1 µg/ml on Hela, HEK29, NIH/3T3 cell lysate and Mouse brain tissue lysate.

Rabbit Polyclonal Antibody to HSP70(Discontinued)
Rabbit Polyclonal Antibody to HSP70(Discontinued)
Rabbit Polyclonal Antibody to HSP70(Discontinued)
Rabbit Polyclonal Antibody to HSP70(Discontinued)

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Figure-1 : Western blot analysis of HSP70 Antibody at 1 µg/ml on  Hela, HEK29,  NIH/3T3 cell lysate and  Mouse brain tissue lysate.
Figure-2 : Flow cytometric analysis of HSP70 Antibody on HEK-293 cells, Shaded histogram represents HSP70 Antibody and open histogram represents isotype control, PE conjugated anti-rabbit IgG used as secondary antibody.
Figure-3 : Immunohistochemical analysis of HSP70 Antibody at 10 ug/ml on human Prostate tissue slide (Paraffin embedded).
Figure-4 : Immunohistochemical analysis of HSP70 Antibody at 10 ug/ml on human Liver tissue slide (Paraffin embedded).

Product code: 45-1039

Clonality : Polyclonal
Application : ELISA
Reactivity : Mouse, Human

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  • Product Info
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Format : Purified
Amount : 40 µg
Isotype : Rabbit IgG
Purification : Immunoaffinity chromatography
Content : 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Storage condition : The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Gene : HSPA1A
Gene ID : 3303
Uniprot ID : P0DMV8
Alternative Name : Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP70-1, HSP70.1
Immunogen Information : Synthetic peptide within the N-terminal of human HSP70 protein

HSP70 is a member of the chaperonin family. It stabilizes existing proteins against aggregation and mediates the folding of newly translated polypeptides in both cytosol and organelles. HSP70 participates in all of these processes through its ability to recognize nonnative conformations of other proteins. It binds extended peptide segments with a net hydrophobic character exposed during translation and membrane translocation or following stress-induced damage. Rabbit Anti-HSP70 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within N-terminal of human HSP70 protein.

ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Immunhistochemistry: 10-15µg/ml
Flowcytometry :1-2 µg/10^6 Cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm, Nucleus, Cytoplasm
Post transnational modification: In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress.
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Rabbit Polyclonal Antibody to HSP70(Discontinued)

Product code: 45-1039
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