Fig. 1: Formalin-fixed, paraffin-embedded Tonsil stained with biotinylated Kappa Light Chain probe followed by anti-biotin Rabbit Recombinant Monoclonal antibody (BTN/2032R).
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Product code: 36-3801
| Clone name : | BTN/2032R |
| Application : | IHC |
| Reactivity : | All species |
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| Alternative Name : | Vitamin B7 or Vitamin H |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Immunogen Information : | Biotinylated sheep immunoglobulin |
It recognizes both the free and protein-conjµgated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
| Content : | 200 µg/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
In situ Nucleic Acid Hybridization; Immunohistochemistry (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
