Anti-Napsin A (Lung Adenocarcinoma Marker) Monoclonal Antibody(Clone: NAPSA/1238 + NAPSA/1239)
Fig. 1: Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238 + NAPSA/1239).
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| Amount : | 100 µg |
| Isotype : | Mouse IgG, kappa |
| Content : | 200 µg/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | NAPSA |
| Gene ID : | 9476 |
| Uniprot ID : | O96009 |
| Alternative Name : | ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA |
| Immunogen Information : | Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary) |
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml for 60 minutes at RT); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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