Anti-CD325 PE Mab(8C11)
Figure 1: Separation of HeLa cells stained using anti-human CD325 (8C11) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD325 APC concentration, black-dashed) in flow cytometry analysis (surface staining).
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|Amount :||0.1 mg|
|Isotype :||Mouse IgG1|
|Purification :||Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.|
|Content :||Formulation : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide|
|Storage condition :||Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.|
Specificity: The mouse monoclonal antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Flow cytometry: Recommended dilution: 1-5 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.