Anti-Cyclin D1 Monoclonal Antibody (Clone:CD1.1)
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Format : | Purified |
Amount : | 0.1 mg |
Isotype : | Mouse IgG1 |
Purification : | Purified by protein-A affinity chromatography |
Storage condition : | Store at 2-8°C. Do not freeze. |
Flow Cytometry Application note: Membrane permeabilization is required.
Immunoprecipitation Recommended dilution: 1 µg/ml
Western Blotting Recommended dilution: 1 µg/ml
Immunohistochemistry Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
Immunohistochemistry (frozen sections) Recommended dilution: 2 µg/ml
Positive tissue: colon
Immunocytochemistry Recommended dilution: 1 µg/ml
ELISA
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus, Cytoplasm, Membrane |
Post transnational modification: | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex. |
Tissue Specificity: | Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. |
BioGrid: | 107067. 101 interactions. |
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