Anti-E. coli K99 pili (Clone K9912-349.2)
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| Amount : | 100 µg |
| Isotype : | Mouse IgG1 |
| Purification : | Preparation: Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Content : | Concentration: Lot Specific Formulation: This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. |
| Storage condition : | This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. |
| Alternative Name : | F5 fimbriae, F5 pilus, K99 fimbriae, K99 antigen |
| Immunogen Information : | Purified E. coli K99 (B44) pili |
Specificity
These antibodies recognize K99+ E. coli. They do not cross-react with K99-negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
Background
Bovine enteric colibacillosis is an economically important disease in neonatal calves caused by enterotoxigenic E. coli (ETEC) which adhere to intestinal cells using pili. Most ETEC isolated from cattle use K99 pili to bind to enterocytes of the small intestine. K99 pili are primarily composed of a repeating 18.2kDa major subunit which is responsible for binding to host glycoprotein receptor, N-glycolylneuraminic acid-GM3. A 7.1kb operon of eight genes on a plasmid encode the pili and other associated proteins responsible for regulation, transport, and assembly of K99 pili.
These antibodies have been qualified for use in ELISA to detect K99+ E. coli.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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