Anti-Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody(Clone: EGP40/2041R)
Fig. 1: Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R).
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| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Content : | 200 µg/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | TACSTD1 |
| Gene ID : | 4072 |
| Uniprot ID : | P16422 |
| Alternative Name : | Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1) |
| Immunogen Information : | Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary) |
Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Western Blot (0.5-1.0µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1µg/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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