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  7. Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)

Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)

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Fig. 1: Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2038).

Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)
Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)
Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)
Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)

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Fig. 1: Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2038).
Fig. 2: Formalin-fixed, paraffin-embedded human Skin stained with gp100 Mouse Monoclonal Antibody (PMEL/2038).
Fig. 3: SDS-PAGE Analysis Purified gp100 Mouse Monoclonal Antibody (PMEL/2038). Confirmation of Integrity and Purity of Antibody.
Fig. 4: Analysis of Protein Array containing more than 19,000 full-length human proteins using gp100 Mouse Monoclonal Antibody (PMEL/2038). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Product code: 36-3155

Clone name : PMEL/2038
Clonality : Monoclonal
Application : ELISA, IHC
Reactivity : Human

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  •  100 µg

  • $199.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : SILV
Gene ID : 6490
Uniprot ID : P40967
Alternative Name : 95kDa melanocyte-specific secreted glycoprotein, M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; Premelanosome protein (PMEL17); Secreted melanoma-associated ME20 antigen; SILV; Silver homolog
Immunogen Information : A recombinant fragment (around aa 376-502) of human SILV protein (exact sequence is proprietary)
Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

ELISA (Use Ab at 2-4µg/ml for coating) (Order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody(Clone: PMEL/2038)

Product code: 36-3155
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