Fig. 1: Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).
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Product code: 36-3769
| Clone name : | DT101+ BC199 + HMB45 |
| Application : | IHC |
| Reactivity : | Human |
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| Gene : | MLANA & SILV |
| Gene ID : | 2315; 6490 |
| Uniprot ID : | Q16655 ; P40967 |
| Alternative Name : | Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; PMEL17 |
| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa + Mouse IgG1, kappa |
| Immunogen Information : | Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
