Anti-IgE Monoclonal Antibody (Clone:BE5)-HRP Conjugated
Figure 1: ELISA analysis of polyclonal human IgE (7 different concentrations: 0.2 - 12.5 ng/ml) using capture antibody 4H10 (1.25 μg/ml) and HRP-conjugated detection antibody BE5 (5 μg/ml).
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| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography. |
| Content : | 0.2 mg/ml, Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Gene : | IGHE |
| Uniprot ID : | P01854 |
| Alternative Name : | Immunoglobulin heavy constant epsilon,Immunoglobulin E |
| Immunogen Information : | Purified human IgE. |
Specificity: The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.
Description: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
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