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  7. Anti-Myofibroblast Marker Monoclonal Antibody(Clone: PR 2D3)

Anti-Myofibroblast Marker Monoclonal Antibody(Clone: PR 2D3)

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Fig. 1: SDS-PAGE Analysis Purified Myofibroblast Marker Mouse Monoclonal Antibody (PR 2D3). Confirmation of Purity and Integrity of Antibody.

Anti-Myofibroblast Marker Monoclonal Antibody(Clone: PR 2D3)
Anti-Myofibroblast Marker Monoclonal Antibody(Clone: PR 2D3)

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Fig. 1: SDS-PAGE Analysis Purified Myofibroblast Marker Mouse Monoclonal Antibody (PR 2D3). Confirmation of Purity and Integrity of Antibody.
Fig. 2: Western Blot Analysis of A431, MCF-7 & HeLa cell lysates using Myofibroblast Marker Mouse Monoclonal Antibody (PR 2D3).

Product code: 36-3718

Clone name : PR 2D3
Clonality : Monoclonal
Application : WB IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Immunogen Information : Crude homogenate of normal human colorectal mucosa
Myofibroblasts are a unique group of smooth muscle-like fibroblasts that play an important role in oncogenesis, inflammation, repair, wound contraction and fibrosis. Like smooth muscle (SM) cells, myofibroblasts contain microfilament bundles and express -SM Actin, the Actin isoform that is present in myoepithelial cells and SM cells and especially abundant in vascular SM cells. Myofibroblasts secrete inflammatory and anti-inflammatory cytokines, chemokines, growth factors and lipid and gaseous inflammatory mediators, as well as extracellular matrix proteins and proteases in most organs and tissues. Besides being temporarily present following tissue injuries and fibrocontractive diseases, myofibroblasts are also present under normal conditions in regions such as the skin, pulmonary septa and periodontal ligaments. Stem cell factor and platelet-derived growth factor (PDGF) are two secreted proteins responsible for differentiating myofibroblasts from embryological stem cells. PR 2D3 reacts with a cell membrane component of cells in the pericrypt sheath; with smooth muscle cells and myofibroblasts. This antibody is considered to be the gold standard for the identification of myofibroblasts.

Western Blot (0.5-1.0µg/ml) Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Myofibroblast Marker Monoclonal Antibody(Clone: PR 2D3)

Product code: 36-3718
*specify in mg or ml

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