Anti-Podoplanin Monoclonal Antibody (Clone:IHC650)
|Amount :||0.5 ml|
|Isotype :||Mouse IgG2a, kappa|
|Purification :||Protein A/G Chromatography|
|Content :||Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide|
|Storage condition :||Store at 2°C - 8°C. Do not freeze.|
Podoplanin is a transmembrane mucoprotein specifically expressed in the endothelium of lymphatic capillaries, while remaining absent from the blood vasculature. The protein is co-localized with VEGFR3/FLT4 in normal skin and kidney. Anti-Podoplanin is useful in the identification of lymphangiomas, Kaposi’s sarcomas, epithelioid mesotheliomas, hemangioblastomas, seminomas, and some angiosarcomas which likely have lymphatic differentiation.
Recommended dilutions: Immunohistochemical analysis: 1:100 - 1:200. However, this need to be optimized based on the research applications.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Post transnational modification:||Cleaved by a metalloprotease within its extracellular (EC) domain, generating a membrane-bound C-terminal fragment (PCTF33) and an extracellular fragment. The resulting membrane-bound C-terminal fragment (PCTF33) is further processed between Val-150 and Val-151 by PSEN1/gamma-secretase generating the intracellular domain of podoplanin (PICD).|
|Tissue Specificity:||Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.|
|BioGrid:||115874. 4 interactions.|