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  7. Anti-SIGLEC1 / CD169 / Sialoadhesin Monoclonal Antibody(Clone: HSn 7D2)

Anti-SIGLEC1 / CD169 / Sialoadhesin Monoclonal Antibody(Clone: HSn 7D2)

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Fig. 1: SDS-PAGE Analysis Purified SIGLEC1 / CD169 Mouse Monoclonal Antibody (HSn 7D2). Confirmation of Integrity and Purity of Antibody.

Anti-SIGLEC1 / CD169 / Sialoadhesin Monoclonal Antibody(Clone: HSn 7D2)

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Product code: 36-3164

Clone name : HSn 7D2
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : SIGLEC1
Gene ID : 6614
Uniprot ID : Q9ZZ2
Alternative Name : CD169; CD169 antigen; Sialic acid binding immunoglobulin like lectin 1; Sialic acid-binding Ig-like lectin 1; Sialoadhesin; Siglec-1; SN
Immunogen Information : Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.
Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjµgate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1,709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells.

Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-SIGLEC1 / CD169 / Sialoadhesin Monoclonal Antibody(Clone: HSn 7D2)

Product code: 36-3164
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