Anti-NRF1 Monoclonal Antibody(Clone: NRF1/2609)
Fig. 1: SDS-PAGE Analysis Purified NRF1 Mouse Monoclonal Antibody (NRF1/2609). Confirmation of Purity and Integrity of Antibody.
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| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells throµgh interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thoµght to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.
Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1µg/ml for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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