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  7. Monoclonal Antibody to Alpha-synuclein (Clone: 2A7)

Monoclonal Antibody to Alpha-synuclein (Clone: 2A7)

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Figure-1: Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to α-synuclein (34-1110), dilution 1:1,000, in red, and costained with rabbit pAb to GFAP (34-1042) dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The α-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.

Monoclonal Antibody to Alpha-synuclein  (Clone: 2A7)
Monoclonal Antibody to Alpha-synuclein  (Clone: 2A7)

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Figure-1: Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to α-synuclein (34-1110), dilution 1:1,000, in red, and costained with rabbit pAb to GFAP (34-1042) dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The α-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.
Figure-2: Western blot analysis of different tissue lysates using mAb to α-synuclein (34-1110), dilution 1:1,000 in green. [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. The strong band at about 15kDa corresponds to α-synuclein protein.

Product code: 34-1110

Clone name : 2A7
Clonality : Monoclonal
Application : WB, IF/ICC, IHC
Reactivity : Human, Rat, Mouse, Cow, Pig

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Format : Purified
Amount : 100 µl
Isotype : Mouse, IgG1
Content : Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : SNCA
Gene ID : 6622
Uniprot ID : P37840
Alternative Name : Non-A beta component of AD amyloid, NACP, Non-A4 component of amyloid precursor
Immunogen Information : Full length human protein with the epitope from amino acids 61-95

WB:1:1,000 IF/ICC: 1:1,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm, Membrane, Nucleus, Cell junction, Secreted
Post transnational modification: Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
Tissue Specificity: Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain.
BioGrid: 112506. 474 interactions.
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Monoclonal Antibody to Alpha-synuclein (Clone: 2A7)

Product code: 34-1110
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