Monoclonal Antibody to human CD46
The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity. It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain.
|Content :||0.5 mg, 0.2 µm protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.|
|Storage condition :||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
FACS Analysis: Antibody M177 stains the extracellular domain of CD46. Immuno Precipitation: 107 cells were lysed and immunoprecipitated with 25 µg M177 antibody and 25 µg protein G-sepharose Western Blot Analysis: A non-reduced sample treatment and SDS-page was used. The band size is 45-70 kDa .
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cytoplasmic vesicle|
|Post transnational modification:||In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.|
|Tissue Specificity:||Expressed by all cells except erythrocytes.|
|BioGrid:||110346. 11 interactions.|