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    7. Monoclonal Antibody to human E-selectin

    Monoclonal Antibody to human E-selectin

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    Monoclonal Antibody to human E-selectin

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    Product code: 10-3556

    Clone name : ENA1
    Clonality : Monoclonal
    Application : IP, IHC-Fr
    Reactivity : Human

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    Size
    Price

    Available Pack Size(s)

    •   1.0 mg

    •  500 µg

    • $2,100.00 

    • $1,500.00 

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    Shipping Info:

    For estimated delivery dates, please contact us at support@abeomics.com

    Download TDS MSDS

    • Product Info
    • Antigen Details
    • Description
    • Application Note
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    • Review   (0)
    Amount : 500 µg
    Isotype : Mouse IgG1
    Content : 0.5 mg 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
    Storage condition : Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Gene : SELE
    Gene ID : 6401
    Uniprot ID : P16581
    Alternative Name : ELAM1, CD62E, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2

    ENA1 reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). The antibody reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.

    For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 µg/ml) in tissue culture medium at 37 °C. As negative control it is advised to use a control murine IgGantibody.

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

    Subcellular location: Cell membrane
    BioGrid: 112301. 16 interactions.
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    Monoclonal Antibody to human E-selectin

    Product code: 10-3556
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