Monoclonal Antibody to human Punctin-1

Monoclonal antibody 1B2-8-14 reacts with Punctin-1 (ADAMTSL-1). Punctin-1 is a secreted molecule resembling members of the family of proteases designated ADAMTS (a disintegrin-like and metalloprotease domain with thrombospondin type I motif). ADAMTS proteases are secreted enzymes that consist of a metalloprotease domain (which provides the catalytic activity) and ancillary domains (which provide substrate, cell or extracellular matrix activity). Punctin-1 lacks the pro-metalloprotease and the disintegrin-like domain typical of this family but contains other ADAMTS domains in precise order including 4 thrombospondin-I repeats. Punctin-1 is an extracellular matrix protein in tissues and deposited into the cell substratum and localized towards the periphery of transfected cells. Punctin-1 and Punctin-2 (ADAMTSL-3) are the first members of a novel family of ADAMTS-like proteins that appear to have important functions in the extracellular matrix. Monoclonal antibody 1B2-8-14 recognizes the spacer domain and reacts with molecular species of 55 kDa or a doublet of 55 kDa and 60 kDa (glycosylated form). Note that proteolysis of Punctin-1 has been described and additional smaller bands may also be obtained.

For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.