Monoclonal Antibody to Ikk Gamma (Clone: ABM15D9)
IKK is a trimeric protein complex consisting of two catalytic subunits, IKKalpha and IKKbeta, and a regulatory subunit, IKKgamma. IKKgamma (also termed NEMO or IKBKG) regulates the kinase activity of IKKalpha/beta. IKKgamma plays a critical role in DNA damaged-induced NF-kappaB signaling in a role independent of its function in the IKK complex. While the biological importance of IKKgamma PTMs (Post-Translational Modifications) has been reported under a variety of stimuli, the detection of PTMs has numerous technical limitations. These limitations include both the transient nature of these modifications, as well as low overall abundance of modified IKKgamma inside the cell.
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
Western blot analysis: 2-4 µg/ml, Flowcytometric analysis: 0.5-1 µg/10^6 Cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Cytoplasm, Nucleus|
|Post transnational modification:||Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity.|
|Tissue Specificity:||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.|
|BioGrid:||114089. 324 interactions.|