Monoclonal Antibody to Ku (p70/p80) (Nuclear Marker)(Clone : KU729)
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
|Purification :||Affinity Chromatography|
|Content :||PE conjugated Ku (p70/p80) Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months.|
Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood); Immunofluorescence (1:50-1:100 for 30 minutes at RT); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Nucleus, Chromosome|
|Post transnational modification:||Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.|
|BioGrid:||108822. 283 interactions.|