Monoclonal Antibody to Human/Mouse TLR9 (Clone : 5G5)
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|Amount :||500 µg|
|Isotype :||Mouse IgG2a|
|Content :||0.5 mg, 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.|
|Storage condition :||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
The monoclonal antibody 10-3504 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.
The monoclonal antibody 5G5 can be used for flow cytometry, Western blotting and immuno assays as detection antibody. Furthermore, the monoclonal antibody 5G5 is useful for immunohistology on frozen and paraffin sections. The monoclonal antibody 5G5 stains RAW macrophages and TLR9 (CD289) transfected HEK293 cells. The antibody is weakly cross reactive with human TLR9.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle|
|Post transnational modification:||Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain (PubMed:18931679, PubMed:18820679). Cleavage requires UNC93B1 (PubMed:18820679). Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling (PubMed:21402738).|
|Tissue Specificity:||Expressed in the basolateral region of gastric epithelial cells with high levels detected in antrum and body mucosa (at protein level). Detected in spleen and stomach at higher levels in C57BL/6 mice than BALB/C.|
|BioGrid:||219897. 1 interactions.|
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