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  7. Polyclonal Antibody to IkB-epsilon (Phospho-Ser22)

Polyclonal Antibody to IkB-epsilon (Phospho-Ser22)

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Figure 1: Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody 35-1199 .

Polyclonal Antibody to IkB-epsilon (Phospho-Ser22)
Polyclonal Antibody to IkB-epsilon (Phospho-Ser22)

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Figure 1: Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody 35-1199 .
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Phospho-Ser22) Antibody 35-1199 (left) or the same antibody preincubated with blocking peptide(right).

Product code: 35-1199

Clonality : Polyclonal
Application : IHC, WB
Reactivity : Mouse, Human

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  •   50 µl

  •  100 µl

  • $349.00 

  • $412.00 

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Format : Purified
Amount : 100 µl
Isotype : Rabbit IgG
Content : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : Nfkbie
Gene ID : 18037
Uniprot ID : O54910
Alternative Name : I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon
Immunogen Information : Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Mouse IkB-e.

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74 Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z.J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488.

Predicted MW: 40kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm
Post transnational modification: Serine phosphorylated; followed by proteasome-dependent degradation.
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Polyclonal Antibody to IkB-epsilon (Phospho-Ser22)

Product code: 35-1199
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