Recombinant Human ACE2 Protein with hFc tag
Fig 1 : Recombinant Human ACE2 Protein with hFc tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 140-150 kDa.
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| Amount : | 100 µg |
| Purification : | >95% by SDS-PAGE. |
| Content : | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. Reconstitution: For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| Storage condition : | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
| AA sequence : | Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a Fc tag at the C-terminus. |
Source: HEK293 cells.
Endotoxin: < 0.1 EU/µg of the protein by LAL method.
Calculated MW : 109.5kDa
Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a Fc tag at the C-terminus.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike RBD-6His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2-Fc, the EC50 of ACE2-Fc is 4-6 ng/mL.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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