TLR5/HEK293 Stable Cell Line
Fig-1: Functional analysis of the TLR5/HEK293 cell line. TLR5/HEK293 cells as well as parental HEK293 cells were transfected with NF-kB/Renilla luciferase reporter plasmid for 16 h. Cells were stimulated with different doses of flagellin (Abeomics, Cat. #15-1014) for 16 h followed by luciferase assay using Luciferase Assay Reagent (Abeomics, Cat. #17-1101).
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Order now and get it on Thursday November 14, 2024
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Amount : | 1 Vial |
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
TLR5/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses human Toll-like receptor 5 (TLR5, also designated as CD285). TLR5 mediates immune responses by recognition of bacterial flagellin, of which activation involves in various human diseases including inflammatory bowel disease, rheumatoid arthritis, osteoclastogenesis, gastric cancer, cervical cancer and ovarian cancer. Note that TLR5 in the TLR5/HEK293 stable cell line contains the N-terminal HA tag (Figure 2), which does not interfere with TLR5 activity as confirmed by functional assay (Figure 1).
Sequence data: Human TLR5 (accession number NP_003259)
Application:.
- Functional assay.
Culture conditions:
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics ([email protected]).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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