Anti-Cytokeratin 19 Monoclonal Antibody (Clone:A53-B/A2)

Product code: 30-1052

Clone name : A53-B/A2
Clonality : Monoclonal
Application : IHC
Reactivity : Human

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0.1 mg

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG2a
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : KRT19
Gene ID : 3880
Uniprot ID : P08727
Alternative Name : KRT19
Immunogen Information : MCF-7 human breast adenocarcinoma cell line

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, positive control: MCF-7, BA-17, HT-29 cells.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Tissue Specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
BioGrid: 110078. 63 interactions.
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