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  7. Monoclonal Antibody to CD43 (Clone: DF-T1)

Monoclonal Antibody to CD43 (Clone: DF-T1)

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Fig.1: Cell Surface flow analysis of anti-hCD43 antibody (36-3009) in PBMC (Lymphocytes gated) using 0.5 µg/10^6 cells. Green represents isotype control (ABEOMICS); red represents anti-hCD43 antibody. Goat anti-mouse PE conjugated secondary antibody was used (ABEOMICS).

Monoclonal Antibody to CD43 (Clone: DF-T1)
Monoclonal Antibody to CD43 (Clone: DF-T1)
Monoclonal Antibody to CD43 (Clone: DF-T1)
Monoclonal Antibody to CD43 (Clone: DF-T1)

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Fig.1: Cell Surface flow analysis of anti-hCD43 antibody (36-3009) in PBMC (Lymphocytes gated) using 0.5 µg/10^6 cells.  Green represents isotype control (ABEOMICS); red represents anti-hCD43 antibody. Goat anti-mouse PE conjugated  secondary antibody was used (ABEOMICS).
Fig. 2: Immunohistochemical analysis of CD43 in human spleen using CD43 antibody (Clone: DF-T1) at 1:200 dilution.
Fig. 3: Immunohistochemical analysis of CD43 in human spleen tissue using CD43 antibody (Clone: DF-T1) at 10 µg/ml.
Figure-4: Western blot analysis of Anti-CD43 antibody in human spleen tissue using 1 µg/ml of Anti-CD43 (DF-T1).

Product code: 36-3009

Clone name : DF-T1
Clonality : Monoclonal
Application : IHC, FACS, WB
Reactivity : Human

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100 µg
$459.00 

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1 Kappa
Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : SPN
Gene ID : 105369261
Uniprot ID : P16150
Alternative Name : SPN, CD43
Immunogen Information : Myeloblastic KG1 cells were used as the immunogen for this antibody.

This antibody recognizes a 95/115/135kDa (depending upon the extent of glycosylation) surface transmembrane glycoprotein, identified as CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein). CD43 is expressed on all thymocytes and T-lymphocytes. Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. CD43 is also found in a range of lymphoid and myeloid tumors. Expression of CD43 may be used to identify B-lymphoblastic lymphoma, since the malignant cells in this condition are often CD43 positive. Since CD43 antibody stains granulocytes and their precursors, this antibody may be used as a marker for myeloid tumors. This antibody is also useful in identification and classification of T-cell malignancies.

Immunohistochemical analysis: 1:200-1:500, FACS analysis: 0.5-2 µg/10^6 cells, Westernblot analysis: 1-2 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Nucleus
Post transnational modification: CD43 cytoplasmic tail: Sumoylated.
Tissue Specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
BioGrid: 112571. 13 interactions.
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Monoclonal Antibody to CD43 (Clone: DF-T1)

Product code: 36-3009
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