Monoclonal Antibody to CD43 (Clone: DF-T1)
|Amount :||100 µg|
|Isotype :||Mouse IgG1 Kappa|
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
This antibody recognizes a 95/115/135kDa (depending upon the extent of glycosylation) surface transmembrane glycoprotein, identified as CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein). CD43 is expressed on all thymocytes and T-lymphocytes. Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. CD43 is also found in a range of lymphoid and myeloid tumors. Expression of CD43 may be used to identify B-lymphoblastic lymphoma, since the malignant cells in this condition are often CD43 positive. Since CD43 antibody stains granulocytes and their precursors, this antibody may be used as a marker for myeloid tumors. This antibody is also useful in identification and classification of T-cell malignancies.
Immunohistochemical analysis: 1:200-1:500, FACS analysis: 0.5-2 µg/10^6 cells, Westernblot analysis: 1-2 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Nucleus, Nucleus|
|Post transnational modification:||CD43 cytoplasmic tail: Sumoylated.|
|Tissue Specificity:||Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.|
|BioGrid:||112571. 13 interactions.|