Recombinant Sars-Cov-2 (COVID-19/2019-nCov) Spike S1 Protein
|Amount :||100 µg|
|Purification :||>95% by SDS-PAGE.|
|Content :||Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.|
|Storage condition :||Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|
|AA sequence :||Tag Fc, 6×His tag at the C-terminus. (Val11-Arg682)|
|Gene ID :||43740568|
|Alternative Name :||S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1,novel coronavirus s1 Protein|
Source: HEK293 cells.
Endotoxin < 1.0 EU/µg of the protein by LAL method.
Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.
Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.