Recombinant Sars-Cov-2 (COVID-19/2019-nCov) Spike S1 Protein
Fig 1 : Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
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| Amount : | 100 µg |
| Purification : | >95% by SDS-PAGE. |
| Content : | Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4. |
| Storage condition : | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
| AA sequence : | Tag Fc, 6×His tag at the C-terminus. (Val11-Arg682) |
| Gene ID : | 43740568 |
| Alternative Name : | S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1,novel coronavirus s1 Protein |
Source: HEK293 cells.
Endotoxin < 1.0 EU/µg of the protein by LAL method.
Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.
Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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