SARS-CoV-2 Spike Protein S1 (RBD) Fc Tag (319-541 aa)
Figure 1 : ACE2 (human)(rec.) (32-190010) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (32-190010) is coated on an ELISA plate at 1 μg/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) (32-190018) is added (starting at a concentration of 500 ng/ml with a two fold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).
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| Amount : | 50 µg |
| Purification : | ≥ 95% (SDS-PAGE) |
| Content : | Lyophilized. Concentration: 1mg/ml after reconstitution. Reconstitution: Reconstitute with 50µl endotoxin-free water. |
| Storage condition : | Short Term Storage +4°C; Long Term Storage -20°C; Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.For maximum product recovery after thawing, centrifuge the vial before opening the cap. Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| AA sequence : | Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 319-541) is fused to the N-terminus of the Fc region of human IgG1. |
| Alternative Name : | 2019-nCoV Spike Protein S1 (RBD), COVID-19 Spike Protein S1 (RBD) |
Source: HEK 293 cells.
SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor angiotensin-converting enzyme 2 (ACE2) present at the surface of epithelial cells.
Biological Activity: Binds to the human ACE2. Endotoxin Content : <0.001EU/µg purified protein (LAL test).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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