SARS-CoV-2 Spike Protein S1 (RBD) Fc Tag (319-541 aa)
Figure 1 : ACE2 (human)(rec.) (32-190010) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (32-190010) is coated on an ELISA plate at 1 μg/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) (32-190018) is added (starting at a concentration of 500 ng/ml with a two fold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).
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|Amount :||50 µg|
|Purification :||≥ 95% (SDS-PAGE)|
|Content :||Lyophilized. Concentration: 1mg/ml after reconstitution. Reconstitution: Reconstitute with 50µl endotoxin-free water.|
|Storage condition :||Short Term Storage +4°C; Long Term Storage -20°C; Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.For maximum product recovery after thawing, centrifuge the vial before opening the cap. Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.|
|AA sequence :||Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 319-541) is fused to the N-terminus of the Fc region of human IgG1.|
|Alternative Name :||2019-nCoV Spike Protein S1 (RBD), COVID-19 Spike Protein S1 (RBD)|
Source: HEK 293 cells.
Biological Activity: Binds to the human ACE2. Endotoxin Content : <0.001EU/µg purified protein (LAL test).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.