Monoclonal Antibody to CD45RO (Clone: C45.1)
Fig.1: Cell Surface flow analysis of hCD45RO in PBMC (Lymphocytes gated) using 0.5 µg antibody per10^6 cells. Green represents isotype control (ABEOMICS); red represents anti-hCD45RO antibody (10-7509). Goat anti-mouse PE conjugated secondary antibody was used (ABEOMICS).
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The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported.
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Immunohistochemical analysis: 1:250-1:500
FACS analysis: 0.5-2 µg/10^6 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane, Membrane raft |
| Post transnational modification: | Heavily N- and O-glycosylated. |
| Tissue Specificity: | Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes. |
| BioGrid: | 111752. 24 interactions. |
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