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  7. Monoclonal Antibody to CD45RO (Clone: C45.1)

Monoclonal Antibody to CD45RO (Clone: C45.1)

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Fig.1: Cell Surface flow analysis of hCD45RO in PBMC (Lymphocytes gated) using 0.5 µg antibody per10^6 cells. Green represents isotype control (ABEOMICS); red represents anti-hCD45RO antibody (10-7509). Goat anti-mouse PE conjugated secondary antibody was used (ABEOMICS).

Monoclonal Antibody to CD45RO (Clone: C45.1)
Monoclonal Antibody to CD45RO (Clone: C45.1)

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Fig.1: Cell Surface flow analysis of hCD45RO in PBMC (Lymphocytes gated) using 0.5 µg antibody per10^6 cells.  Green represents isotype control (ABEOMICS); red represents anti-hCD45RO antibody (10-7509). Goat anti-mouse PE conjugated  secondary antibody was used (ABEOMICS).
Fig: 2 Immunohistochemical analysis of CD45RO in human tonsil using CD45RO antibody (Clone: C45.1) at 1:500 dilution.

Product code: 10-7509

Clone name : C45.1
Clonality : Monoclonal
Application : IHC, FACS
Reactivity : Human

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Order now and get it on Tuesday January 19, 2021

Same day delivery FREE on San Diego area orders placed by 1.00 PM

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Same-day delivery in San Diego available for the following zip codes:

92121, 92122, 92037, 92117, 92109, 92110, 92101, 92010, 92011, 92009, 92008
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Available Pack Size(s)

  •   25 µg

  •  100 µg

  • $125.00 

  • $295.00 

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Order now and get it on Tuesday January 19, 2021

Same day delivery FREE on San Diego area orders placed by 1.00 PM

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG2a Kappa
Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : PTPRC
Gene ID : 5788
Uniprot ID : P08575
Alternative Name : PTPRC, CD45
Immunogen Information : Interleukin-2-dependent human T lymphocytes were used as the immunogen for the CD45RO antibody.

The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported.

 Immunohistochemical analysis: 1:250-1:500 

FACS analysis: 0.5-2 µg/10^6 cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cell membrane, Membrane raft
Post transnational modification: Heavily N- and O-glycosylated.
Tissue Specificity: Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
BioGrid: 111752. 24 interactions.
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Monoclonal Antibody to CD45RO (Clone: C45.1)

Product code: 10-7509
*specify in mg or ml

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